Enzymic desulphation of porphyran

Enzymic desulphation of l-serine O[S]-sulphate: the intracellular localization of the enzyme.

Desulphation of dextran sulphate during kidney ultrafiltration.

The renal clearance of [3H]dextran sulphate by the isolated perfused rat kidney was associated with desulphation of the molecule, as demonstrated by ion-exchange and affinity chromatography of material resident in both glomeruli and urine samples. This process also occurred in vivo. The molecular size distribution of glomerular dextran sulphate in the perfused kidney was indistinguishable from ...

Enzymic Catalysis

The time of greatest sensitivity to radiation is during the period of organogenesis in the first twelve weeks of human foetal development. Irradiation of the adult can cause life shortening or increased incidence of leukemia. The last chapter is concerned with the basis and origin of radiation protection standards, the hazards of nuclear power plants, high altitude supersonic aerospace travel, ...

Enzymic degradation of heparin.

A sulphamidase and a sulphoesterase were isolated from adapted cells of Flavobacterium heparinum. These enzymes were partially purified from the 'heparinases' present in the bacterial extracts and characterized. The sulphamidase has a high specificity for glucosamine N-sulphate and glucosamine 2,6-disulphate. The activity decreases sharply with increasing molecular weight ofthe substrates teste...

Simulations of enzymic reactions.

A fundamental problem in biology is the explanation of enzyme activity at the molecular level. The detailed origin of the rate acceleration of chemical reactions by enzymes is not fully understood, in spite of the immense amount of experimental work that has been done in this area [1,2]. T o describe an enzyme-catalysed reaction, its mechanism must be firmly established and intermediates must b...